RNA endotoxin hydrophobic interaction ionic recognition multimodal chromatography plasmid DNA. Capto PlasmidSelect is a chromatography resin that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms. The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Cytiva HiTrap Capto PlasmidSelect For the purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications Manufacturer: Cytiva 17549911 View more versions of this product Catalog No. Capto PlasmidSelect is a chromatography resin that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms. Capto Adhere proved to offer a very strong binding of nucleic acids. Columns packed with Capto PlasmidSelect are suitable for purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Stable in up to 1 M NaOH, 40% Isopropanol, up to 1 M acetic acid, and up to 70% ethanol.Ģ0% ethanol at temperatures between 4☌ and 30☌.Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. Hydrophilic properties of base matrix prevent non-specific binding.īioProcess resin supported for industrial applications.Ĭonvenient small-scale purification, process development, and scale-up using prepacked HiTrap columns. Capto PlasmidSelect is produced with robust manufacturing procedures, and can withstand standard cleaning-in-place (CIP) and sanitization-in-place methods. Higher throughput for improved productivity and process economy. Capto PlasmidSelect is also available in bulk for scale-up to manufacturing scale (Fig 4). High-flow purification of supercoiled DNA from research scale to cGMP production.įlexible process design due to a large operational window of flow velocities and bed heights. Capto PlasmidSelect is available in bulk as well as in formats suitable for process development. The possibility to run at higher flow rates and bed heights increases flexibility in process design and allows for an increased productivity. Compared with its predecessor PlasmidSelect Xtra resin, Capto PlasmidSelect is based on a more rigid base matrix, delivering excellent pressure-flow properties to plasmid production. The resin is designed for purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications. Cytiva HiTrap Capto PlasmidSelect For the purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications 497.00 - 965. Capto PlasmidSelect is a chromatography resin that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.Ĭapto PlasmidSelect is a thiophilic aromatic adsorption chromatography resin with a selectivity that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
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